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Protocols for myocardial differentiation of iPS cells co-cultured with HGF and mechanical stretching stimulation system. a Myocardial differentiation protocol using iPS cells co-cultured with HGF. b Protocol for the collection of human gingival tissue and isolation of HGF, HGF cultured on day 11 was shown. c <t>Polydimethylsiloxane</t> <t>(PDMS)</t> stretch chamber.
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Protocols for myocardial differentiation of iPS cells co-cultured with HGF and mechanical stretching stimulation system. a Myocardial differentiation protocol using iPS cells co-cultured with HGF. b Protocol for the collection of human gingival tissue and isolation of HGF, HGF cultured on day 11 was shown. c <t>Polydimethylsiloxane</t> <t>(PDMS)</t> stretch chamber.
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Dow Corning polydimethysiloxane (pdms) chamber sylgard 184 silicone elastomer
Protocols for myocardial differentiation of iPS cells co-cultured with HGF and mechanical stretching stimulation system. a Myocardial differentiation protocol using iPS cells co-cultured with HGF. b Protocol for the collection of human gingival tissue and isolation of HGF, HGF cultured on day 11 was shown. c <t>Polydimethylsiloxane</t> <t>(PDMS)</t> stretch chamber.
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Protocols for myocardial differentiation of iPS cells co-cultured with HGF and mechanical stretching stimulation system. a Myocardial differentiation protocol using iPS cells co-cultured with HGF. b Protocol for the collection of human gingival tissue and isolation of HGF, HGF cultured on day 11 was shown. c <t>Polydimethylsiloxane</t> <t>(PDMS)</t> stretch chamber.
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STREX Inc elastic pdms chambers stb-ch-4w
Protocols for myocardial differentiation of iPS cells co-cultured with HGF and mechanical stretching stimulation system. a Myocardial differentiation protocol using iPS cells co-cultured with HGF. b Protocol for the collection of human gingival tissue and isolation of HGF, HGF cultured on day 11 was shown. c <t>Polydimethylsiloxane</t> <t>(PDMS)</t> stretch chamber.
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Dow Corning pdms chamber sylgard 184
Protocols for myocardial differentiation of iPS cells co-cultured with HGF and mechanical stretching stimulation system. a Myocardial differentiation protocol using iPS cells co-cultured with HGF. b Protocol for the collection of human gingival tissue and isolation of HGF, HGF cultured on day 11 was shown. c <t>Polydimethylsiloxane</t> <t>(PDMS)</t> stretch chamber.
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STREX Inc elastic pdms chambers
Protocols for myocardial differentiation of iPS cells co-cultured with HGF and mechanical stretching stimulation system. a Myocardial differentiation protocol using iPS cells co-cultured with HGF. b Protocol for the collection of human gingival tissue and isolation of HGF, HGF cultured on day 11 was shown. c <t>Polydimethylsiloxane</t> <t>(PDMS)</t> stretch chamber.
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STREX Inc pdms chambers
Protocols for myocardial differentiation of iPS cells co-cultured with HGF and mechanical stretching stimulation system. a Myocardial differentiation protocol using iPS cells co-cultured with HGF. b Protocol for the collection of human gingival tissue and isolation of HGF, HGF cultured on day 11 was shown. c <t>Polydimethylsiloxane</t> <t>(PDMS)</t> stretch chamber.
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pdms chambers - by Bioz Stars, 2026-07
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Protocols for myocardial differentiation of iPS cells co-cultured with HGF and mechanical stretching stimulation system. a Myocardial differentiation protocol using iPS cells co-cultured with HGF. b Protocol for the collection of human gingival tissue and isolation of HGF, HGF cultured on day 11 was shown. c Polydimethylsiloxane (PDMS) stretch chamber.

Journal: Scientific Reports

Article Title: Effect of mechanical stretching stimulation on maturation of human iPS cell-derived cardiomyocytes co-cultured with human gingival fibroblasts

doi: 10.1038/s41598-025-16063-9

Figure Lengend Snippet: Protocols for myocardial differentiation of iPS cells co-cultured with HGF and mechanical stretching stimulation system. a Myocardial differentiation protocol using iPS cells co-cultured with HGF. b Protocol for the collection of human gingival tissue and isolation of HGF, HGF cultured on day 11 was shown. c Polydimethylsiloxane (PDMS) stretch chamber.

Article Snippet: All samples were cultured using a PDMS (polydimethylsiloxane) stretch chamber (size: 20 × 20 mm, Menicon, Aichi, Japan) to apply mechanical stretching stimulation during the induction of differentiation into cardiomyocytes (Fig. a and c).

Techniques: Cell Culture, Isolation